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Figure. Influence of gel stiffness on OPC differentiation. (a–c) OPCs (oligondrocyte precursor cells) and OLs (adult oligodendrocytes) were labeled against A2B5 and O4 protein markers and the morphology was assessed using fluorescence microscopy. Morphology was categorized as (a) simple, (b) complex, and (c) membranous; scale bars 25 um. (d) The percentage of cells in the 3 categories grown on PAAm gels and on glass. (e) Cell area quantified from the fluorescence images and (f) percentage of MBP-expressing cells as a function of substratum stiffness. Error bars are SEM; *P < 0.05, **P < 0.01,***P < 0.001.